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±¸°­¿¡¼­ ºÐ¸®ÇÑ E. duransÀÇ S. mutans¿Í S. oralis¿¡ ´ëÇÑ ÀÛ¿ë

INTERACTION OF ORAL ENTEROCOCCUS DURANS WITH STREPTOCOCCUS MUTANS AND STREPTOCOCCUS ORALIS

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Abstract

kabstract :
À屸±ÕÀº »ç¶÷ÀÇ ±¸°­, Áú, Àå³» µî¿¡ Á¤»óÀûÀ¸·Î Á¸ÀçÇÏ´Â ¼¼±ÕÀÌ´Ù. º» ¿¬±¸¿¡¼­´Â ±¸°­
¿¡¼­ ºÐ¸®µÈ À屸±ÕÁß Enterococcus durans·Î µ¿Á¤µÈ ºÐ¸®±ÕÁÖ 4ÁÖÀÇ Æ¯¼º°ú ±¸°­³» ÁÖ¿ä
¼¼±ÕÀÎ Streptococcus mutans¿Í Streptococcus oralis¿ÍÀÇ °ü°è¸¦ ¿¬±¸ÇÏ¿© ´ÙÀ½°ú °°Àº °á
°ú¸¦ ¾ò¾ú´Ù.
1. ºÐ¸®±ÕÁÖ¿¡ ´ëÇÑ ´ç ¹ßÈ¿ °Ë»ç¿Í »ýÈ­ÇÐÀû °Ë»ç °á°ú, ºñ½ÁÇÑ ¾ç»óÀ» º¸¿´´Ù.
2. ºÐ¸®±ÕÁÖ ¸ðµÎ erythromycin, penicillin, tobramycin, ampicillin, telcoplanin,
ciprofloxacin, vancomycin, gentamicin, kanamycin ¹× streptomycin¿¡ ´ëÇØ °¨¼ö¼ºÀ» º¸¿´
´Ù.
3. S.mutans¸¦ ÀÏȸ¿ë Å¥º¦¿¡¼­ ´Üµ¶ ¹è¾ç½Ã 550§¬¿¡¼­ÀÇ Èí±¤µµ°¡ 1.405À̾úÀ¸³ª,
S.mutans¿Í 4ÁÖÀÇ ºÐ¸®±ÕÁÖ È¥ÇÕ¹è¾ç½Ã¿¡´Â °¢°¢ÀÇ Èí±¤µµ°¡ 0.855, 0.867, 0.797, 1.083À¸·Î
°¨¼ÒµÇ¾ú´Ù.
4. ºñÄ¿ ¿ÍÀÌ¾î °Ë»ç °á°ú S. mutans ´Üµ¶ ¹è¾ç½Ã Çü¼ºµÈ ÀΰøÄ¡ÅÂÀÇ Æò±Õ ¹«°Ô´Â 1566¡¾
103§·À̾ú´Ù. S.mutans¿Í 4ÁÖÀÇ ºÐ¸®±ÕÁÖ È¥ÇÕ ¹è¾ç½Ã¿¡´Â °¢°¢ 44¡¾5§·, 41¡¾12§·, 34¡¾7
§·, 38¡¾12§·À¸·Î ÇöÀúÈ÷ °¨¼ÒµÇ¾ú´Ù. ¹è¾çÈÄ »ý±Õ¼ö´Â S.mutans ´Üµ¶ ¹è¾ç½Ã §¢´ç 2.0¡¿
109À̾úÀ¸³ª, S.mutans¿Í 4ÁÖÀÇ ºÐ¸®±ÕÁÖ È¥ÇÕ ¹è¾ç½Ã S.mutans´Â 2.0¡¿
107 ³»Áö´Â 6.0¡¿107À¸·Î °¨¼ÒµÇ¾ú´Ù.
5. S.oralis ´Üµ¶ ¹è¾ç½Ã §¢´ç 2.1¡¿108À̾úÀ¸³ª, S.oralis¿Í 4ÁÖÀÇ ºÐ¸®±ÕÁÖ
È¥ÇÕ ¹è¾ç½Ã S.oralis ´Â 1.4¡¿107 ³»Áö 7.0¡¿107À¸·Î °¨¼ÒµÇ¾ú
´Ù.
6. 3ÁÖÀÇ ºÐ¸®±ÕÁַκÎÅÍ ¾à 60kbÀÇ plasmid¸¦ ºÐ¸®ÇÒ ¼ö ÀÖ¾ú´Ù.
ÀÌ»óÀÇ °á°ú¸¦ Á¾ÇÕÇÏ¸é ±¸°­¿¡¼­ ºÐ¸®µÈ E.durans´Â S.mutansÀÇ Áõ½ÄÀ» ¾ïÁ¦ÇÏ¿© Àΰø
Ä¡Å Çü¼ºÀ» ÀúÁöÇÏ¿´°í, S.oralisÀÇ Áõ½ÄÀº ¾à°£ ¾ïÁ¦ÇÏ¿´´Ù.
#ÃÊ·Ï#
-Abstract-
Enterococcus is a normal inhabitant of the human oral cavity, the vagina, and the
gastrointestinal tract. Four isolates of Enterococcus in this study were identified as
E.durans. These bacteria were characterized and the interaction of these bacteria with
the important oral bacteria as like S.mutans and S.oralis was studied as follows.
1. The carbohydrate fermentation test and biochemical test showed similar results in 4
isolates.
2. The susceptibility test against erythromycin, penicillin, tobramycin, ampicillin,
teicoplanin, ciprofloxacin, vancomycin, gentamicin, kanamycin, and streptomycin showed
to be susceptible in all four isolates.
3. The optical density of absorbance at 550 nm was 1.405 in the culture of S.mutans
in disposable cuvette, whereas being 0.855, 0.867, 0.797 and 1.083 in the combined culture
of S.mutans and each E.durans.
4. The mean weight of produced artificial plaque on the wires in the beaker was 1566
¡¾103§· in culture of S.mutans only, whereas being reduced to 44¡¾5§·, 41¡¾21§·, 34¡¾7
§·, and 38¡¾12§· in the combined culture of S.mutans and each E.durans. The viable
cells were 2.0¡¿109 per §¢ in the culture of S.mutans, whereas being 2.0¡¿
107 to 6.0¡¿107 per §¢ in the combined culture of S.mutans
and E.durans.
5. The viable cells were 2.1¡¿108 per §¢ in the culture of S.oralis,
whereas being 1.4¡¿107 to 7.0¡¿107 per §¢ in the combined
culture of S.oralis and E.durans.
6. Plasmid of about 60 Kb was isolated in three isolates of E.durans.
These results suggested that E.durans isolated from the oral cavity inhibited the
replication of S.mutans and formation of artificial plaque, while inhibiting the replication
of S. oralis a little.

Enterococcus durans; Streptococcus mutans; Streptococcus oralis; Enterococcus durans; Streptococcus mutans; Streptococcus oralis;

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